Nanion Introducing: SyncroPatch 384PE - the PatchEngine. Superior Ion Channel Drug Screening. - PDF

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Nanion Introducing: SyncroPatch 384PE - the PatchEngine. Superior Ion Channel Drug Screening. The PatchEngine a Revolutionary Instrument for APC-based Ion Channel Drug Screening Executive summary 384 or
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Nanion Introducing: SyncroPatch 384PE - the PatchEngine. Superior Ion Channel Drug Screening. The PatchEngine a Revolutionary Instrument for APC-based Ion Channel Drug Screening Executive summary 384 or even 768 cells in parallel (modular approach) Up to 20,000 data points per day Reliable data from diverse ion channels targets Integrates easily into an HTS environment Cost per data point: 0,2 Available for shipping NOW! The PatchEngine a Revolutionary Instrument for APC-based Ion Channel Drug Screening Key features 384 or 768 cells in parallel (384 or 768 amplifier) 384 pipettor head 85 % success rates routinely obtained Excellent results with voltage gated & ligand gated ion channels Modular approach easy upgrade & integration Single- and multi-hole recording substrates SyncroPatch 384 PE Set-up overview Components: SyncroPatch 384 PE Hardware 1-2 x 384 Channel Amplifier Biomek FX with a 384 pipetting head SyncroPatch 384 Software NPC-384 Borosilicate Chip Temperature Controlled Cell Hotel 384 Pipette Tips (Beckmann) Open design allows for seamless integration into robotic HTS-highways SyncroPatch 384 PE Consumables NPC-384 Available as single and multi-hole plates: Single-hole substrate: Four-hole substrate: Data Control 384 Outstanding data handling and export DataControl 384 intuitive & easy-to-use featuring: Intuitive, quick & easy-to-use setup of exps Powerful data analysis functions Compound view Automated Data Export PDF-reporting Raw data traces of 384 hkv1.3 current responses SyncroPatch 384 PE Assay compatibility examples The SyncroPatch 384 PE has been validated with: CHO, HEK, LTK and RBL cells A variety of voltage gated ion channels A variety of ligand gated ion channels See the following data examples: Current Voltage Relationship of hkv1.3 expressed in CHO cells Success rate: 89.6 % CHO-hKv1.3 cells were kindly provided by Evotec Current Voltage Relationship of hkv1.3 expressed in CHO cells Success rate: 89.6 % CHO-hKv1.3 cells were kindly provided by Evotec hkv1.3 block by Quinidine Success rate: 85.7 % CHO-hKv1.3 cells were kindly provided by Evotec hkv1.3 block by Quinidine (5 µm, 50 µm and 500 µm ) Success rate: 85.7 % CHO-hKv1.3 cells were kindly provided by Evotec Current Voltage Relationship of hnav1.7 expressed in CHO cells Success rate: 90.9 % CHO-hNav1.7 cells were kindly provided by Anaxon Current Voltage Relationship of hnav1.7 expressed in CHO cells Success rate: 90.9 % CHO-hNav1.7 cells were kindly provided by Anaxon hnav1.7 block by Tetracaine (5 µm, 50 µm and 500 µm ) Success rate: 92.4 % CHO-hNav1.7 cells were kindly provided by Anaxon hnav1.7 block by Tetracaine (5 µm, 50 µm and 500 µm) Success rate: 86.2 % CHO-hNav1.7 cells were kindly provided by Anaxon Potassium Currents through the Endogenes Kir 2.1 in RBL cells Success rate: 94.8 % The Fluidic Properties of the SyncroPatch 384 PE were tested on RBL cells. The external solution was changed from low KCl to high KCl and back. Potassium Currents through the Endogenes Kir 2.1 in RBL cells Success rate: 94.8 % The Exchanges Rates are below 100 ms for wash in times as well for wash out times. Dose Response Curve of Glycine on the human α 1 -Glycine Receptor Success rate: 83.3 % LTK cells with human α 1 -Glycine Receptor 100 µm, 300 µm and 1 mm Glycine applied Using multi-hole chip Cells were kindly provided by AstraZeneca. hgaba A α 1,β 2,γ 2 expressed in HEK cells Success rate: 81.5 % Three applications of 5 µm GABA Using multi-hole chip SyncroPatch 384PE Superior Ion Channel Drug Screening Giga-seal recordings 384 or even 768 simultaneous whole-cell recordings Multiple units per liquid handler High success rates - 85 % Voltage- and ligand gated ion channels Competitive price per data point ~ 0,20 Customized hole sizes and multi-hole chips Up to data points day Customized to your HTS environment Nanion Customer Reference - a global customer base distributed between industrial and academic laboratories Companies: Abbott Almirall Prodesfarma AstraZeneca Amgen Aurigene Discovery Technologies, Ltd. Axiogenesis Bayer Bionomics Boehringer Ingelheim Bristol-Myers Squibb Center for New Drug Safety Evaluation and Research (NCDSER), Chromocell conogenetix biosciences GmbH Evotec Genionics GlaxoSmithKline Grünenthal Hofman La Roche Hydra Biosciences Japanese Tobacco Lupin Limited Merck Merch Serano Novartis Novo Nordisk Nycomed Ono Pharmaceutical Orion Pharma Pfizer Pierre Fabre, Regeneron Pharmaceuticals Rytmion Sanofi-Aventis Shinogi Siena Biotech SK Holding, Co Ltd Sundia MediTech Company, Ltd. Suven Life Sciences Ltd, Hyderabad, India Syngenta UCB Pharma Vertex Pharmaceuticals Vimta Labs Life Sciences, Hyderabad, India Wyeth Xention Research institues and universites: Cambridge University, Cambridge, UK Charité - Universitätsmedizin Berlin, Consiglio Nazionale delle Ricerche, Povo (Trento), Italy Copenhagen University, Denmark Cornell University, Geneva, NY, USA Eberhard Karls Universität Tübingen, FMP, Berlin, Forschungszentrum Caesar, Bonn, Forschungszentrum Jülich GmbH, Jülich, Friedrich-Schiller-Universität Jena, Georg-August-Universität Göttingen, Griffith University, Nathan, Australia GSF - Forschungszentrum für Umwelt und Gesundheit, GmbH, Neuherberg, Hasselt University, Diepenbeek,Belgium Hertie Institute for Clinical Brain Research, Howard Florey Institute, USA Howard Hughes Medical Institute, Ashburn, VA, USA Institute of Genomics and Integrative Biology, Delhi, India IPBS-CNRS, Tolouse, France Istituto Italiano di Tecnologia, Genova, Italy Jacobs University, Bremen, JNCASR, Bangalore, India Julius von Sachs Institut, Würzburg, Karolinska Institute, Stockholm, Sweden Kiserleti Orvostudomanyi Kutatointezet, Budapest, Hungary Korea Institute of Science and Technology (KIST), Seoul, South Korea Laboratorio Especial de Toxinologia Aplicada CAT-CEPID, Sao Paolo, Brazil Ludwig Maximillian Universität, München, Lund University, Lund, Sweden Max Planck Institut, Göttingen, Medizinische Hochschule Hannover, Research institues and universites: (cont.) Nanjing University, Nanjing, China Nanyang Technological University, Singapore National University of Singapore, Singapore National Center for New Drug Safety Evaluation and Research (NCDSER), Shanghai, China National Heart Center, Singapore National Jewish Medical and Research Center, Denver, CO, USA NIH/NINDS, Bethesda, MD, USA NIST, Gaithersburg, MD, USA New York University, NY, USA RMIT University, Bundoora, Australia Ruhr-Universität Bochum, Shanghai Institute of Materia Medica, Shanghai, China Simon Fraser University, Burnaby, Canada Southampton General Hospital, Southampton, UK Stiftung Alfred-Wegener-Institut für Polar- und Meeresforschung, Bremerhaven, Syracuse Univerity, NY, USA Technische Universität Ilmenau, Technische Universität Darmstadt, Technische Universiät München, Uppsala University, Uppsala, Sweden UNESCO, Bahía Blanca, Argentina Universidade Federal de Sao Paulo, Brazil Università degli Studi di Genova, Italy Universitatea Lucian Blaga Din Sibiu, Romania University of California, Los Angeles, CA, USA University of California, San Diego, CA, USA University of Cambridge, UK University of Cincinnati, OH, USA University of Groningen, the Netherlands University of Leeds, UK Universidade de Lisboa, Portugal Research institues and universites: (cont.) University of Michigan, Ann Arbor, MI, USA Università degli Studi di Napoli - Federico II, Napoli, Italy University of North Carolina, Wilmington, NC, USA University of Queensland, Brisbane, Australia University of Rochester, NY, USA University of Tennessee Health Science Center, USA University of Valladolid and Spanish National Research Council (CSIC), Valladolid, Spain University of Washington, USA University of Wisconsin, Madison, WI, USA Universität Zürich, Switzerland Universitatea Lucian Blaga din Sibiu, Romania Universität Augsburg, Universität Freiburg, Universität für Bodenkultur, Wien, Austria Universität Heidelberg, Universität Hohenheim, Universität Osnabrück, Vanderbilt Medical School, Nashwille, TN, USA Virginia State University, Blacksburg, VA, USA X-Lab, Göttingen, Yale University, New Haven, CT, USA Inje University, Busan, South Korea
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